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samples: samples.csv
ref:
species: "Drosophila melanogaster"
genome: ""
annotation: ""
accession: "GCF_000001215.4"
ensembl_species: "" # e.g., "homo_sapiens"
build: "" # e.g., "GRCh38"
release: "" # e.g., "105"
read_filter:
min_length: 200
minimap2:
index_opts: ""
opts: ""
maximum_secondary: 100
secondary_score_ratio: 1.0
samtools:
samtobam_opts: "-b"
bamsort_opts: ""
bamindex_opts: ""
bamstats_opts: ""
quant:
salmon_libtype: "U"
deseq2:
fit_type: ""
design_factors:
- "condition"
lfc_null: 1.0
alt_hypothesis: "greaterAbs"
point_width: 20
mincount: 10
alpha: 0.05
threshold_plot: 10
colormap: "Blues"
figtype: "png"
batch_effect:
- ""
isoform_analysis:
FLAIR: true
qscore: 1
exp_thresh: 10
col_opts: "--annotation_reliant generate --generate_map --stringent"
protein_annotation:
lambda: false
uniref: "https://ftp.imp.fu-berlin.de/pub/lambda/index/lambda3/gen_0/uniref50_20230713.lba.gz"
num_matches: 3
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This workflow performs differential expression analysis of RNA-seq data obtained from Oxford Nanopore long-read sequencing technology.
First a transcriptome FASTA is constructed using gffread [https://github.com/gpertea/gffread]. Reads are then mapped to the transcriptome with the long-read optimized alignment tool minimap2 [https://github.com/lh3/minimap2].
Next quantification is performed using salmon [https://github.com/COMBINE-lab/salmon] before normalization and differential expression analysis are conducted by PyDESeq2 [https://github.com/owkin/PyDESeq2].
The workflow can optionally analyze splice-isoforms through integrating the FLAIR [https://github.com/BrooksLabUCSC/flair] workflow.
Additionaly, NanoPlot [https://github.com/wdecoster/NanoPlot] is employed to analyze initial sequencing data and QualiMap [https://github.com/EagleGenomics-cookbooks/QualiMap] is used to evaluate mapping results.
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https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/WorkflowJob
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/hasOutput
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-7-alignment_qa_report
https://w3id.org/np/snakemake/hasOutput
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alignment_qa_report
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-70-sample_qa_plot
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-71-sample_qa_plot
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https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-72-sample_qa_plot
https://w3id.org/np/snakemake/hasOutput
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sample_qa_plot
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-73-sample_qa_plot
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-73-sample_qa_plot
https://w3id.org/np/snakemake/hasOutput
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sample_qa_plot
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-74-sample_qa_plot
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/hasOutput
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-75-standardize_gff
https://w3id.org/np/snakemake/hasOutput
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https://w3id.org/np/snakemake/ruleName
standardize_gff
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/hasOutput
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-76-total_sample_qa_plot
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-77-transcriptid_to_gene
https://w3id.org/np/snakemake/hasOutput
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https://w3id.org/np/snakemake/hasOutput
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
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https://w3id.org/np/snakemake/hasOutput
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alignment_qa_report
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-9-alignment_qa_report
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
https://w3id.org/np/snakemake/WorkflowJob
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/job-9-alignment_qa_report
https://w3id.org/np/snakemake/hasOutput
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alignment_qa_report
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from workflow configuration
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https://w3id.org/np/snakemake/hasConfigurationFile
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/assertion
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/assertion
http://www.w3.org/ns/prov#wasAttributedTo
https://orcid.org/0000-0003-2408-7588
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/pubinfo
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://purl.org/dc/terms/created
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://purl.org/dc/terms/creator
https://orcid.org/0000-0003-2408-7588
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://purl.org/nanopub/x/hasNanopubType
https://schema.org/Dataset
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://purl.org/nanopub/x/signedBy
https://orcid.org/0000-0003-2408-7588
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://www.w3.org/2000/01/rdf-schema#label
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://www.w3.org/ns/prov#generatedAtTime
2026-04-17T17:19:48.418353
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
http://www.w3.org/ns/prov#wasAttributedTo
https://orcid.org/0000-0003-2408-7588
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/sig
http://purl.org/nanopub/x/hasAlgorithm
RSA
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/sig
http://purl.org/nanopub/x/hasPublicKey
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/sig
http://purl.org/nanopub/x/hasSignature
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https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/sig
http://purl.org/nanopub/x/hasSignatureTarget
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE
https://w3id.org/np/RAK9xz_ccnu0Xhs4vX2KtqCxX44mmSt6nq-ePLeewMrFE/sig
http://purl.org/nanopub/x/signedBy
https://orcid.org/0000-0003-2408-7588