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MINSEQE describes the Minimum Information about a high-throughput nucleotide SEQuencing Experiment that is needed to enable the unambiguous interpretation and facilitate reproduction of the results of the experiment. By analogy to the MIAME guidelines for microarray experiments, adherence to the MINSEQE guidelines will improve integration of multiple experiments across different modalities, thereby maximising the value of high-throughput research. Metadata collection template includes critical information such as: 1) The description of the biological system, samples, and the experimental variables being studied, 2) The sequence read data for each assay, 3) The ‘final’ processed (or summary) data for the set of assays in the study, 4) General information about the experiment and sample-data relationships, and 5) Essential experimental and data processing protocols.
NOTE: The provided resource documentation at Zenodo contains version 1.0 of the MINSEQE guidelines, which originated from discussions at the March 2008 Functional Genomics Data (FGED) Society-hosted workshop held in Berkeley, CA, USA. The FGED Society was operational from 1999-2021.
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